Paper Title
Influence of Organism Aging on Metabolomic Profile Created from Blood
Abstract
Aging (senescence) represents probably the greatest disease of all. There is very little known about it and it is
indeed inevitable. There are generally two main explanations of aging – genetic programming and oxidative damage. The
both alternatives are widely explored via number or variously designed animal studies. Our model organism were birds (barn
swallows) because of their very fast metabolism that is similar to the human one. The analytical methods based on liquid
chromatography combined with mass spectrometry were developed to monitor levels of antioxidants and to create agedependent
metabolic profile. There was found out, that in the period of three years the amount of antioxidants drops by
minimally 10%. Moreover, the amount of biomarkers of oxidative damage to organism rises over the same period of time by
15% in average. These findings support the theory of oxidative damage as a key mechanism of aging.
Keywords - Aging, oxidative stress, antioxidants, metabolomics, senescence, mass spectrometry