Paper Title
Green Synthesis and Characterization of Iron Nanoparticles using Aesculus hippocastanum Seed Extract

Aesculus hippocastanum (horse chestnut) seed extract was used for iron nanoparticles (FeNPs) synthesisin this study. FeNPs were produced and stabilized due to the biomaterials that are found in the content of the seed extract. Particlecharacterization was analyzed by Transmission electron microscopy (TEM), Energy - dispersive X-ray Spectroscopy (EDX),UV- Visible spectroscopy, Dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR). Zeta potentialand pH were measured. TheUV-Visible, XRD, FTIR and analysis results showed that FeNPs were produced in oxideform. Absorption peaks at the wavelength of 205 nm and 291 nm indicated the formation of hydrolysis products of metallic iron (Fe0). FTIR, XRD and EDX analysis showed signals due to the oxide and oxyhydroxide iron nanoparticles. TEM images clearly showed that the nanoparticles were in spherical shape mostly with 9 ± 7nm diameters and were seen as polydisperse. The hydrodynamic diameter of the nanoparticles, that are surrounded by biologic substances adsorbed on the surface, was measured from 50.75 nm to 712.4 nm.Colloid stability was determined as moderate according to 25.5 mV value which was in the range of ± 20 – 30 mV. pH was measured as 6.47 and 2.2 before and after the nanoparticle synthesis respectively. Keywords - Iron Nanoparticles, Green Synthesis, Green Nanotechnology, Aesculus hippocastanum