One-Step Purification of Lysozyme by Ion Exchange Nanofibrous Membrane in a Stirred Cell Contactor
Polyacrylonitrile nanofiber membranes (PAN) were fabricated via electrospinning technique. The PAN membrane
comprises a polyethylene terephthalate (PET) spunbond fabric as a supporting layer with upper and lower PAN nanofibrous
membrane. After NaOH and HCl treatment procedures, the modified membrane is a weak ion exchange membrane (namely
AEA-COOH). The AEA-COOH membrane was characterized in terms of fiber diameter, porosity, specific area, pore size,
ionic density, and binding capacity. In this study, lysozyme was chosen as a model protein. The adsorption efficiency of the
AEA-COOH ion exchange membrane for lysozyme was assessed by the measurements of breakthrough curves in a small
stirred cell system (Model Millipore 8010). The capture of lysozyme from diluted chicken egg white (CEW) was carried out
with a working volume of 10 mL under the different feed concentrations, flow rates, rotating speeds, and operating pressures.
The optimal adsorption conditions were found to be adsorption pH9, 1 in 10 dilution of CEW, average flow rate of 0.1 mL/min,
rotating speed of 200 rpm, and initial operating pressure of 0 psi). Furthermore,the elution of lysozyme wasalso investigated in
stirred cell experiments. It was successful in achieving purification of lysozyme in a high yield of 98.2% with a purification
factorof 62.9 in a single step.
Index Terms - Chicken egg , electrospinning technique ,lysozyme , Polyacrylonitrile nanofiber membranes