Paper Title
Antioxidant Activity Fraction of Kaffir Lime Leaves (Citrus Hystrix Dc.) By 1,1-Diphenyl-2-Picrylhydrazil (DPPH) Radical Scavenging Method

The sample used in this study is the leaves of Citrus hystrix DC. belong to the family Rutaceae. This research aimed to determine the antioxidant activity of Citrus hystrix DC. leave fraction using 1,1-diphenyl-2-picrylhydrazil (DPPH) free radical scavenging method. Citrus hystrix DC. leaf extract was extracted by maceration using ethanol 96% and obtained ethanol extract yield value of 5,044% and subsequently fractionated by Vacuum Liquid Chromatography (VLC) with the mobile phase is n-hexane:ethyl acetate and ethyl acetate:methanol. The stationary phase used silica gel F254. The number of fractions obtained are 4 fractions. The results showed that the Citrus hystrix DC. leave fraction has antioxidant activity. The value of IC50 fraction 1 is 275,814 μg/mL, fraction 2 is 200,297 μg/mL, fraction 3 is 242,247 μg/mL and fraction 4 is 121,831 μg/mL. The 4thfraction of the Citrus hystrix DC. leave has higher antioxidant than the other fractions. Index terms - Antioxidant, Citrus hystrix DC., kaffir lime leaves, 1,1-diphenyl-2-picrylhydrazil (DPPH), Vacuum Liquid Chromatography (VLC).