Paper Title
Tris(Hydroxymethyl)Aminomethane Affinity Membrane Foradsorption of Lysozyme

Polyacrylonitrile (PAN) nanofiber membrane was prepared by electrospinning technique. The PAN membrane used in this work comprises a polyethylene terephthalate (PET) spunbond fabric as a supporting layer with upper and lower PAN nanofiber membrane. After 3 M NaOH and diluted HCl treatments, the weak cationic exchange membrane (i.e., P-COOH) was obtained. The P-COOH membrane was then functionalized with tri-s(hydroxymethyl) aminomethane as an affinity nanofiber membrane (i.e., P-Tris). In this study, lysozyme was chosen as a model protein. The physical properties of the nanofiber membranes were characterized in terms of fiber diameter, porosity and pore size, specific area of the surface, FTIR and SEM analysis. The adsorption experiments were carried out in a well-mixed system under the various operating conditions (e.g., modification pH, adsorption pH, and the molar ratio of reactants, P-COOH/Tris). The dynamic adsorption characteristics of the P-Tris membranes for lysozyme by membrane chromatography were assessed by measurements of the breakthrough curves. The influences of operating conditions (e.g., adsorption pH, lysozyme concentration, no. of sheet membrane, and flow rate) on the adsorption performance of membrane were investigated in an AKTA prime chromatographic system (GE Healthcare). Index Terms - Polyacrylonitrilenanofiber,lysozyme,Tris, AKTA