Morphometric Analysis of Hepatocytes between Acute and Chronic Diabetic Stages
Diabetes mellitus (DM) affects throughout body system including hepatobiliary system. Hepatocyte, an important
hepatic parenchymal cell, is also disturbed. Consequently, the hepatic metabolism impairs and leading to severe hepatopathy.
The aim of this study was to investigate the parameter changes of hepatocyte, nucleus, and nucleolus in diabetic rats by using
morphometric analysis technique. Forty male adult Sprague-Dawley rats were used. Diabetic group (acute: n=10 and
chronic: n=10 stages) were intraperitoneally injected with 60 mg/kg/BW of streptozotocin (STZ). Each control rats (acute:
n=10 and chronic: n=10 stages) were injected with citrate buffer at pH 4.5 via the same route. Animals were sacrificed at
acute (4 weeks) and chronic (24 weeks) periods. In diabetic group, whole blood glucose and urine glucose levels increased.
Hepatic tissues were processed in traditional histological technique. Samples were examined under a light microscope and
analyzed morphometric parameters of mononuclear hepatocyte in periportal (Z1) and pericentral (Z3) zones by using
UTHSCSA Image tool (IT) 3.0 software. Mean areas of hepatocyte, nucleus, and nucleolus were increased in zone 1 and
zone 3 of acute DM rats. Whereas, mean areas of hepatocyte, nucleus, and nucleolus were significantly reduced in both
zones of chronic diabetic rats. Moreover, binuclear hepatocytes were increased in acute DM while they were significantly
decreased in chronic diabetic rats. In conclusion, hepatic morphometric alterations occurred in DM rats that imitate diabetic
patients. This data contributes the severity of diabetic hepatocytes with untreated insulin for further clinical research.
Keywords - Morphometric Analysis, Hepatocyte, Diabetes Mellitus,