Probiotic Potential, Antibacterial And Antioxidant Activities Of Lactobacillus Plantarum CM1
This study evaluated the probiotic potential, antibacterial and antioxidant activity of Lactobacillus plantarum CM1 isolated from cow milk collected from Udaipur city. The probiotic potential includes the tolerance to bile and acid, resistance to antibiotics. Tolerance to bile and acid was determined by growing them in the presence of three different bile salts and simulated gastric juice with different pH values respectively. This strain was tested for resistance against 7 different antibiotics. The antibacterial activity of the culture supernatants neutralized with and without NaOH was tested against different gram-positive and negative pathogenic bacteria. Antioxidant activity was determined in terms of total antioxidant activity by two in-vitro methods i.e. ferric reducing antioxidant power (FRAP) assay and Linolenic acid (LA) test. From the results obtained Lactobacillus plantarum CM1 showed tolerance to all the 3 bile salts namely sodium taurocholate, oxgall and sodium tauroglycocholate used in the study. Among all the three bile salts Lactobacillus plantarum CM1 showed better growth in the presence of oxgall while fair growth in presence of sodium taurocholate and less growth in presence of sodium tauroglycocholate. This strain showed considerable survival at all the pH values used in the study for 3h but best survival was observed at pH 7 after 3h. Among 7 different antibiotics the culture showed resistance against only two antibiotics namely vancomycin and kanamycin. This strain also showed demonstrable antibacterial activity in both the supernatants neutralized with and without NaoH against all the pathogenic bacteria used in the study. Lactobacillus plantarum CM1 showed good antioxidant activity in both the tests. In FRAP assay the reducing power observed for this strain was 514.45 µM FeSO4 and in LA test high inhibition of linolenic acid peroxidation was observed which was 32.80%. The findings of the present study suggested that this strain may be a promising candidate for use as a probiotics.
Keywords: antibacterial activity, antibiotics, antioxidant activity, bile salts, Lactobacillus