Paper Title
Analytical Method Development And Validaton Of RP HPLC For Simultaneously Estimation Of Chlorpheniramine Maleate, Dextromethorphen Hydrobromide, Phenylephrine Hydrochloride In Cough Syrup

Abstract
It is simple, accurate, precise, rapid, selective and reproducible reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of Dextromethorphen Hydrobromide, Phenylephrine Hydrochloride of Chlorpheniramine Maleate in Cough syrup. The first method of three drugs which involves absorbance measurement at 265 nm. Linearity was obtained in the range of 5-15 μg/ml, 2.5-7.5 μg/ml and 1-3 μg/ml respectively. Good Chromatographic separation was achieved isocratically at 35 o C ± 0.5o C on Partisil 10 SCX column (250 × 4.6 mm i.d.) with a mobile phase composed of Buffer: Methanol the ratio of 40:60 % V/V at flow rate of 1.0ml/min. The retention time of Phenylephrine Hydrochloride, Dextromethorphen Hydrobromide, and Chlorpheniramine Maleate retention time was found to be 5.0 min, 8.0 min., and 13.0min respectively. The correlation coefficient for calibration curve of all three peaks was found to be 0.9999. The developed methods were validated according to ICH guidelines. Keywords- Dextromethorphen Hydrobromide, Phenylephrine Hydrochloride of Chlorpheniramine Maleate, HPLC, Validation.