<strong>Paper Title</strong><br>
RAPD-PCR Based Analysis of Genetic Variation Induced in Triticum Aestivum Under Chromium Stress<br>
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<strong>Abstract</strong><br>
Plants acts as a bioindecator to evaluate the effects of pollution on environment. The purpose of present study was
to analyze the phenotypic change in wheat under chromium stress at molecular level. Three concentrations of
chromium10ppm, 40ppm and 80ppm were used to study their effects of plant growth, 80ppm limit the growth and induce
dwarfness in plants. Dwarf plants were subjected to RAPD analysis using ten primers among those only three primers A, B
and C were reproducible. Appearance of new bands and disappearance of normal bands in gel shows the change in the
DNA .Primer B and C shows 100% polymorphism, while primer C produces 50% polymorphism. Similarity coefficient
range from 0 to 0.756 were found in between W1 and W2. This indicates that 80ppm chromium is able to bring mutagenic
effects in the wheat.
Key Words- Chromium, RAPD ,DNA, Toxicity ,parts per million (ppm)