Paper Title
Isolation and Characterization of Active Components Derived from Whole Plant of Ionidium Suffruticosum (GING.)

Abstract
The scope of the present investigation was to isolate the active components present in whole plant of Ionidium suffruticosum. The plant were extracted with various solvents (pet. ether, ethyl acetate and methanol), methanol was found to be more active among them. The preliminary phytochemical results revealed that coumarins, flavonoids and amino acids as active constituents in methanolic extract of Ionidium suffruticosum. The methanolic extract of Ionidium suffruticosum was undergone column chromatography with different solvent fractions. Despite, two compounds were isolated from methanolic extract of Ionidium suffruticosum with the compound 1 was eluted with benzene: Chloroform 70:30, v/v and compound 2 was eluted with ethyl acetate: methanol, 80:20, v/v. The structures of the two isolated compounds were characterized by using FT-IR, NMR and Mass spectrophotometric methods. Thus, the compound 1 was characterized as 3-amino-6-hydroxy- 4-(4-methylphenyl)-2H-chromen-2-one (C 16 H 13 NO 3 ) and the compound 2 was characterized as 1-amino-1-ethoxypropan-2- ol (C 5 H 13 NO 2) . Obviously, this is the first report of occurrence of 3-amino-6-hydroxy-4-(4-methylphenyl)-2H-chromen-2- one in nature as well as the amino ester in this plant. Furthermore, pharmacological studies required for the isolated compounds. Key words- Ionidium suffruticosum, column chromatography, FT-IR, NMR.