Paper Title :Heavy Metal Accumulation by Saccharomyces Cerevisiae Cells Expressing Cup1cystein-Rich Olygopeptides Targeted to The Inner Face of The Plasma Membrane

Author :Ileana Cornelia Farcasanu, Aurora Daniela Neagoe, Lavinia Liliana Ruta

Article Citation :Ileana Cornelia Farcasanu ,Aurora Daniela Neagoe ,Lavinia Liliana Ruta , (2019 ) " Heavy Metal Accumulation by Saccharomyces Cerevisiae Cells Expressing Cup1cystein-Rich Olygopeptides Targeted to The Inner Face of The Plasma Membrane " , International Journal of Advances in Science, Engineering and Technology(IJASEAT) , pp. 39-44, Volume-7,Issue-4

Abstract : Cup1 is the Saccharomyces cervisiaemetallothionein responsible for buffering the surplus copper taken up by yeast cells. In this study we obtained S. cerevisiae cells armed with three metal-binding oligopeptides derived from Cup1 sequence targeted to the inner face of the plasma membrane. The three metal binding oligoppetidescorrespon to three cystein-rich metal-binding sequences (MeBSeq1-3) from Cup1 primary stucture. We describe the constructionof S. cerevisiaestrains expressing MeBSeq1-3 fused to the carboxy-terminus of amyristoylated green fluorescent protein (myrGFP). These strains were investigated in terms of tolerance to heavy metals exposure and heavy metal accumulation. Keywords - Heavy Metal, Saccharomyces Cerevisiae, Cup1, Metal-Binding Sequence

Type : Research paper

Published : Volume-7,Issue-4

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