Paper Title :Semi-Quantitative Analysis of Nitric Oxide Production in Three-dimensional Glioblastoma Multiforme Models in Vitro

Author :Elena Afrimzon, Yana Shafran, Naomi Zurgil, Maria Sobolev, Mordechai Deutsch

Article Citation :Elena Afrimzon ,Yana Shafran ,Naomi Zurgil ,Maria Sobolev ,Mordechai Deutsch , (2019 ) " Semi-Quantitative Analysis of Nitric Oxide Production in Three-dimensional Glioblastoma Multiforme Models in Vitro " , International Journal of Advances in Science, Engineering and Technology(IJASEAT) , pp. 71-76, Volume-7,Issue-4

Abstract : Glioblastoma Multiforme (GBM) remains an incurable brain disease. One of the most important signaling molecules in the brain, nitric oxide (NO) mediates important inter- and intracellular interaction in astrocytes and neurons. In the presented study, the three-dimensional glioblastoma multicellular models were established from A-172 cells and grown in vitro in a picowell array. "Intra-spheroid" NO content and its association with mitochondrial function were studied at a resolution of single 3D object. The association between "intra-spheroid" levels of nitric oxide and mitochondrial status is revealed in real-time measurements and may serve as a significant target for tumor cure. Keywords - Glioblastoma Multiforme, Nitric Oxide, Mitochondrial Membrane Potential, Three-Dimensional Multicellular Models in Vitro

Type : Research paper

Published : Volume-7,Issue-4

	|		PDF	|	Viewed - 48	|	Published on 2020-01-24