Paper Title :Determination Of Selected Pesticide Residues In Fruits Using Quechers Approach And Reversed-Phase High Performance Liquid Chromatography (RP-HPLC)

Author :Sanjan Choudhary, Nitu Bhatnagar

Article Citation :Sanjan Choudhary ,Nitu Bhatnagar , (2016 ) " Determination Of Selected Pesticide Residues In Fruits Using Quechers Approach And Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) " , International Journal of Advances in Science, Engineering and Technology(IJASEAT) , pp. 19-22, Volume-4,Issue-1

Abstract : This paper presents the application of reverse phase high performance liquid chromatographic (RP-HPLC) method equipped with UV detector for the determination of five pesticides, in common fruit samples. The reverse phase liquid Chromatography were used for the monitoring of pesticide residues employing QUEChERS approach of selected organophosphate pesticide Monocrotophos, 2.4 D, Chlorpyrifos, Malaxon, Isopruton, and Phorate sulphoxide. Proposed method was successfully applied for the determination of pesticide residues in orange, apple, pomegranate and grape fruits. Average recoveries achieved for all the pesticides at fortification levels of 1, 0.5, 0.1 mg/l in analyzed fruits were above 90% with relative standard deviations (RSD) less than 6%. The correlation coefficients (r) were higher than 0.999 indicating a high level of linear relationship between the concentration of monochrotophos and peak area. Index Terms- Reversed-Phase High Performance Liquid Chromatography (RP-HPLC), Pesticides Residue, Quechers Approach, Correlation Coefficient.

Type : Research paper

Published : Volume-4,Issue-1

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