Paper Title :Isolation, Characterization and Identification of DNA’S Lactic Acid Bacteria (LAB) of Dadih Lintau (Tanah Datar District) which is Antibiotic Resistance
Author :Endang Purwati, James Hellyward, Yuherman, Hendri Purwanto, Puji Hartini R
Article Citation :Endang Purwati ,James Hellyward ,Yuherman ,Hendri Purwanto ,Puji Hartini R ,
(2018 ) " Isolation, Characterization and Identification of DNA’S Lactic Acid Bacteria (LAB) of Dadih Lintau (Tanah Datar District) which is Antibiotic Resistance " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 28-31,
Volume-6, Issue-2
Abstract : Research about isolation, characterization, and identification of DNA’s lactic acid bacteria of dadih Lintau Tanah
Datar District which is antibiotic resistant has been done. Eleven isolates were isolated; U4, U5, U6, U7, U8, U9, U10, U11, U12, U13
and U14; characteristic of isolates are broken white, round shape, convex, edge slick and Gram positive bacteria. Nine (9)
isolates are rod cell, 2 others are cocci. isolates were characterized based on antibiotic resistance. Ampicillin 10 μg (AM 10),
Diclixacillin 1 μg (DCX1), Gentamicin 10 μg (CN 10), Cofeperazone 75μg (CFP 75), Carbenicillin 100μg (CB 100) have been
used as antibiotics test. The result showed that six isolates resistance to all of antibiotics; U4, U5, U6, U7, U8 and U11. The
result of DNA identification by primer 27F-1429R, showed that PCR product about 1500 base pair. BLAST analisys result
from all dadih Lintau isolate include the lactic acid bacteria group, match 97% - 100% with Lactobacillus plantarum.
Keyword - Antibiotic, Buffalo Milk, Dadih, Fermented, Lactic Acid Bacteria
Type : Research paper
Published : Volume-6, Issue-2
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-11997
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Published on 2018-06-29 |
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