Paper Title :In Vitro Behavior of Zn-Modified Calcium Phosphate Fine Powders
Author :Boyka Andonova-Lilova, Radostina Alexandrova, Kostadinka Sezanova, Rumiana Gergulova, Diana Rabadjieva
Article Citation :Boyka Andonova-Lilova ,Radostina Alexandrova ,Kostadinka Sezanova ,Rumiana Gergulova ,Diana Rabadjieva ,
(2019 ) " In Vitro Behavior of Zn-Modified Calcium Phosphate Fine Powders " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 1-6,
Volume-7,Issue-1
Abstract : Zinc modified (Zn/(Zn+Ca) = 0, 0.03, 0.13) calcium phosphate fine powders were synthesized by biomimetic
approach and subsequent high-temperature treatment. Bi-phase mixture of β-tricalcium phosphate (β-TCP) and
hydroxyapatite (HA) was prepared at non modified sample and monophase Zn-β-TCP at Zn modified samples. The in vitro
biological behavior of the prepared powders was studied using human Lep-3 and bovine MDBK cells. The effect of the
materials on cell viability and proliferation was evaluated by three methods with different molecular / cellular targets and
mechanisms of action - MTT test, neutral red uptake assay and crystal violet staining. The results obtained revealed high
viability of both Lep-3 and MDBK cells cultured for 72h and 96h in the presence of the materials examined applied as 100%
material-modified cell culture medium in all three cases. The dilution of material-modified cell culture medium with nonmodified
culture medium leads to increasing of cell viability.
Type : Research paper
Published : Volume-7,Issue-1
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-14944
View Here
Copyright: © Institute of Research and Journals
|
|
| |
|
PDF |
| |
Viewed - 74 |
| |
Published on 2019-04-23 |
|