
<strong>Paper Title</strong><br>
Simultaneous Analysis Of Taurine And Histamine In Fishes Samples<br>
<br>

<strong>Abstract</strong><br>
This study investigated the taurine and histamine content of  some  fishes  that sold  in Thailand. Quantitative 
analysis of  taurine and histamine were studied by high-performance liquid chromatography using fluorescence detection 
(HPLC-FLD) and derivatization with pre-column o-phthalaldehyde (OPA).  The method of the analysis was validated. The 
correlation coefficients (R2
) of taurine and histamine were 0.9975 and 0.9980 respectively. Limits of detection were 0.001 
mg L-1
 for taurine and 0.03 mg L-1
 for histamine. Limits of quantitation were 0.005 mg L-1
 for taurine and 0.05 mg L-1
 for 
histamine. The method was showed high sensitivity, selective and rapid for the determination of taurine and histamine. The 
method was applied to quantity of taurine and histamine in fish samples. Taurine in Thai fishes were found in the range of 
490.81-2,180.88 mg kg-1
, and the highest level  of taurine were found in Barramundi Giant Seaperch. Histamine in Thai 
fishes were  found in the range of 7.41-19.07 mg kg-1
, and the highest level of histamine were found in Red Mullet. This 
work proved that both taurine and histamine could be separated and detected in the same time, so this modified method also 
used to analyse taurine and histamine in fishes samples. 
 
Keywords— Taurine, Histamine, high-performance liquid chromatography, seafish, freshwater fish.