Paper Title :Bioremediation of Soil with High Heavy Metal Concentration, at Laboratory Scale, Using Native Microorganisms from Mining Waste in Colquijirca, Peru
Author :J. Ramos, A. Llanos, B. Champe, O. Soto
Article Citation :J. Ramos ,A. Llanos ,B. Champe ,O. Soto ,
(2024 ) " Bioremediation of Soil with High Heavy Metal Concentration, at Laboratory Scale, Using Native Microorganisms from Mining Waste in Colquijirca, Peru " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 87-95,
Volume-12,Issue-1
Abstract : This study aims to evaluate the bioremediation potential of indigenous microorganisms from areas impacted by
mining. Following this approach, two soil samples were extracted from mining sites in Colquijirca, Pasco. Subsequently, 1
kg of each sample was sent to the CEIMIC S.A.C laboratory for mass spectrophotometry analysis to determine the initial
concentration of all heavy metals present in the samples. Prior to the isolation of native soil microorganisms, a preenrichment
was conducted to ensure the presence of Bacillus, Pseudomonas, and fungi. Two strains with potential growth
were identified: strain CP-11 (extracted from a culture medium for Bacillus sp.) and CP-08 (extracted from a culture medium
for Pseudomonas sp.), which were isolated and cryo-preserved. Then, using bioaugmentation technique, biomass broths were
prepared for each strain. For the laboratory-scale bioremediation stage, two soil systems were prepared with the collected
samples to simulate the piles of mining waste material. Soil system 1 and soil system 2 were treated with the bioaugmented
solutions of Bacillus sp. and Pseudomonas sp., respectively. The treatment lasted for one month with constant mechanical
ventilation. Finally, a mass spectrophotometry analysis was conducted for each post-treatment sample, determining that the
removal efficiency of CP-11 in sample 1 for heavy metals (As, Cu, Pb, Hg, Cr, and Cd) was 8.2%, 29.6%, 56.8%, 57.2%,
20.8%, respectively. Similarly, the elimination efficiency of CP-08 in M2 for the same metals was 12.2%, 22.7%, 52.8%,
13.2%, 76.6%, and 36.3%, respectively.
Keywords - Bioremediation, native microorganisms, mining-affected soils, heavy metals, bioaugmentation, removal
efficiency, mass spectrophotometry.
Type : Research paper
Published : Volume-12,Issue-1
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-20615
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Published on 2024-06-06 |
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